2016 F-150 with keyless start button that will not work inside or outside mygarage when parked overnight.

Remove the CL-330 from the property and see if that fixes the problem.

That would be "her" CL-330 so I might as well move my truck off the property - good answer and I will try - same thing as I have the Ext Auto-start attached to the key fob and I will separate. By the way, putting the fob in the over-ride slot does nothing when this occurs. Only auto-start can start the vehicle.

Does the problem affect both remotes if you have two, the other being on the second set of keys? Will the system work if you get closer to the vehicle outside? Usually problems with radio signal transmitters are the signals are being blocked by some construction used in the building of a building where the signal cannot get out (like some cellphones while inside buildings) or another signal from a different transmitter source interfering with the signal from your hand held transmitter. Try moving the vehicle away from the house in short increments then testing. Although wifi internet connections should not affect your system, who knows, there may be a little internet geek boosting his signal so Martians can get a free connection.

OJ - yes, the problem affects both remotes. System will not work regardless of where I am when it is non functional. I'm curious to see if (1) the garage door opener signal may be an issue but again that's not always by any means. The one factor that seems a constant is when the car sits for 36-48 hours its a guarantee not to start. Just overnight and it will almost always start.?????

Spoke to a GM mechanic about the problem and I am relating his comments. The remote start feature should be interlaced with the passive anti-theft system in the vehicle. Meaning if the PCM detects a fault or signal in any of the components within that system the remote start function will not work. He suggest when the non start conditions occurs try cycling all systems in the anti- theft system to on, off, open, closed and see if the system will reset. He also stated there should be an open hood sensor which will prevent the car from starting when it is detected the hood is open, this is a safety feature protecting mechanics. There is usually an "open door/door ajar" feature which will disable the system. This sensor could be misaligned or separating from its contact due to metal expansion or deformation when the weather changes. Basically, since the system is computer driven, the fault condition cannot be determined unless the fault is present when the technician plugs in his diagnostic equipment and reads the fault from PCM readout. You can also manually open and close all the doors to see if the problem corrects itself.