My 2003 land cruiser temp gauge is heating up all of a sudden coolant is fine what should I look for?

You will need to look at the following, check to see if the fans are turning on when the reaches operating temperature. Check the fuse for the fan. Your radiator cap may be defective. You could be low on fluid in your reserve reservoir. Your radiator maybe short some antifreeze. Thermostat could be defective. Water pump may be starting to go. Your fan sensor could be defective not allowing the fans to come on.

If it overheats at highway speeds, like 55 mph+, it's not fan(s), they don't run anyway, are redundant. The air-flow at speed is plenty. If you mean at idle or traffic, then yes, could be fans and follow joemoms advice..it's good. Thermostat is best bet to start with. But are you talking about it getting in to the red or just say, ¾ up the gauge?

My land cruiser wise pip is out from reditor and ac to start.